ISHS Acta Horticulturae 110: V International Symposium on Virus diseases of Ornamental Plants
HONEYSUCKLE LATENT VIRUS, A CARLAVIRUS INFECTING LONICERA PERICLYMENUM AND L. JAPONICA (CAPRIFOLIACEAE) |
| Authors: | A.A. Brunt, S. Phillips, B.J. Thomas |
| Abstract:
Honeysuckle latent virus (HLV), a hitherto undescribed carlavirus, was found in S.E. England infecting Lonicera japonica and L. periclymenum, but was not detected in L. nitida or five related genera (Leycesteria, Sambucus, Symphoricarpus, Viburnum and Weigelia). HLV was transmissible in the non-persistent manner by the aphid Hydaphis foeniculi, and by sap-inoculation to 8 of 22 species from 4 of 8 families. It was best cultured in Nicotiana clevelandii and assayed in C. murale. The virus is moderately stable in vitro; sap from N. clevelandii was infective after dilution to 1/1,000 but not 1/10,000, after 10 min at 80 but not 85°C, and after 4–8 days at 20°C or 32–64 days at 2°C. HLV has filamentous particles mostly c. 13 x 650 nm which sediment as a single component with a sedimentation coefficient of 157 S, and contain c. 5% single-stranded RNA of mol. wt 2.8 million and 95% protein of a single polypeptide of mol. wt 31,000. These properties place HLV in the carlavirus group; it is serologically distantly related to red clover vein mosaic and poplar mosaic viruses, but is probably unrelated to 14 other members of the group. | |
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