ISHS Acta Horticulturae 110: V International Symposium on Virus diseases of Ornamental Plants
DETECTION OF A WIDE SPECTRUM OF VIRUS STRAINS BY INDIRECT ELISA AND SEROLOGICAL TRAPPING ELECTRON MICROSCOPY (STREM) |
| Authors: | M.H.V. Van Regenmortel, A. Nicolaïeff, J. Burckard |
| Abstract:
Several authors have reported that ELISA and immunoelectron microscopy are highly specific procedures which often fail to detect serologically related virus strains. Since the extent of serological cross-reactivity between many strains of tobacco mosaic virus is known with a considerable degree of precision, this system was used to determine how closely related serologically two strains must be 1) in order to be trapped on electron microscope grids coated with heterologous antiserum (STREM) and 2) in order to be detected by indirect ELISA. When the serological differentiation index between two strains is higher than about 4, the cross-reactivity is too low to obtain sufficient trapping on grids coated with heterologous antiserum. In such a case, however, the indirect ELISA with antiglobulin conjugates is still capable of detecting the heterologous strain. Since antibodies prepared in different animal species were required, chickens, rabbits and goats were used for immunization. The chicken antibody was prepared from egg yolk obtained from immunized laying hens. | |
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