ISHS Acta Horticulturae 1099: II International Symposium on Horticulture in Europe
EXPERIENCES OF BIOLOGICAL CONTROL OF PSEUDOMONAS VIRIDIFLAVA ON CUT FLOWERS OF RANUNCULUS ASIATICUS |
| Authors: | S. Fascella, G.L. Boeri, B. Cangelosi, C. Pasini, M. Benuzzi , P. Curir |
| Keywords: | Bacillus amyloliquefaciens, Bacillus subtilis, leaf rot, Ranunculus asiaticus, Reynoutria sachalinensis |
| DOI: | 10.17660/ActaHortic.2015.1099.33 |
| Abstract:
The main pathology affecting buttercup (Ranunculus asiaticus) crops of the Riviera Ligure is the infection of the epigeal organs, caused by Pseudomonas viridiflava. It is a disease of bacterial origin which affects plant leaves and causes the appearance of internerval and stem browning necrotic spots and the subsequent decaying of leaves with further collapse of the tissues. When environmental conditions are favourable, the development of disease may also involve flowers. We report the results of three trials of fight against P. viridiflava on cut flowers cultivar ‘Pink Elegance’ in climatic chamber at 6-8°C and humidity close to saturation. In all the trials, flowers were inoculated artificially with a suspension of the pathogen, at the concentration of 1×108 cfu (colony forming units)/ml. In the first trial: copper penta-hydrate 5.5%, (Mastercop), copper hydroxide 26.2%, (Heliocuivre), Fosetyl-Al 25% + oxychloride copper 25%, (R6 Bordeaux), acibenzolar-S-methyl 50%, (Bion 50 WG), copper sulphate neutralized with lime 20%, (Selecta Disperss), Bacillus subtilis strain QST 713, 1.34%, (Serenade ASO) were used in post-inoculation. In the following two trials: copper hydroxide 19.9% (Heliocuivre S), tribasic copper sulphate 15.2% (Cuproxat), Bacillus amyloliquefaciens strain 747 25%, ≥ containing 437.5×1012 cfu/hl (Amylo-X), B. amyloliquefaciens strain 747 25% ≥ containing 312.5×1012 cfu/hl (Amylo-X), Reynoutria sachalinensis extract 5%, (Regalia), have been evaluated, respectively. These products were administered at different times: pre-inoculation and post-inoculation of the bacterium. In summary, the use of biological active ingredients allowed an effective control of P. viridiflava, especially when they were administered in the pre-inoculation phase. The extract of R. sachalinensis showed good ability to limit infection in pre-inoculation and post-inoculation, better than the copper-based products. The effect of B. subtilis and B. amyloliquefaciens, that in some cases displayed a good efficacy in controlling the disease, needs further investigation. | |
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