ISHS Acta Horticulturae 109: III International Symposium on Flower Bulbs
RECENT ADVANCES IN RESEARCH ON LILY SYMPTOMLESS VIRUS |
| Authors: | T.C. Allen, O. Ballantyne, J. Goodell, W.C. Anderson, W. Lin |
| Abstract:
Lily symptomless virus (LSV)-free hybrid lilies have been excised from bulb scale pieces on tissue culture medium. Early excision of scale bulblets more than doubled the number of LSV-free plants of "Red Carpet". In contrast, this porcedure did not produce LSV-free plants of Lilium longiflorum cvs., "Ace" and "Nellie White". These varieties were freed of LSV only after shoot tip apex culture. Thirty-one native lilies representing 25 species were collected in Oregon and California then assayed for LSV. Leaf extracts did not contain LSV, even when obtained from native lilies growing near LSV-infected commercial lilies. Our usual method of LSV detection, electron microscopy of extracts, was compared to enzyme-linked immunosorbent assay (ELISA). Initial results indicated that these assay methods were equally effective for lilies relatively heavily infected with LSV. Antisera from The Netherlands, Germany, and Oregon reacted in ELISA with our isolates of LSV. | |
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