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ISHS Acta Horticulturae 109: III International Symposium on Flower Bulbs

PROPAGATION OF HYACINTH BY LEAF CUTTINGS. HISTOLOGICAL CHANGES IN LEAF CUTTINGS OF HYACINTHUS ORIENTALIS

Author:   J. Krause
Abstract:
A histological study of the leaf cuttings of 'Lady Derby' and 'L'Innocence' indicated that both adventitious roots and new bulbils arose at the basal part of cuttings soon after putting leaves into the rooting media.

Root formation was an endogenous process; parenchyma cells of the vascular system and cells lying close to it formed the root primordia. These cells divided very rapidly. New roots grew paralell to veins and finally emerged through the callus at the base of the cuttings. Adventitious roots were formed very intensively during 4 to 5 weeks after excision of cuttings.

Simultaneously, new bulbils were formed. It was an exogenous process, which started a few days after preparation of cuttings and lasted about 6 weeks. The bulb primordia arose from cells of epidermal or subepidermal levels. Most of the new bulbils were formed at the base of the inner surface of the leaves. Bulb primordium became differentiated in scales. The first, outermost scale, was very small and open. The next, usually two scales, were closed and surrounded the moristem.

The number and size of adventitious bulbs was dependent on the cultivar.

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