ISHS Acta Horticulturae 109: III International Symposium on Flower Bulbs
THE GROWTH OF TULIP TISSUES IN VITRO |
| Authors: | N.A. Wright, P.G. Alderson |
| Abstract:
Large numbers of adventitious shoots have been induced to form on floral stem sections of tulip cv. Merry Widow cultured in vitro, using a modified Murashige & Skoog medium containing inorganic salts and vitamins at full strength and supplemented with casein hydrolysate, benzylamino-purine (BAP) and naphthyl acetic acid (NAA). Between 70% and 90% of floral stem explants regularly produced shoots on this medium. In comparison with other explants from the bulb (scale and axillary bud), floral stem tissue showed the greatest potential for shoot production. The stage of development of the bulb was found to be an important factor in determining the ability of explants to regenerate shoots. Shoot producing potential of floral stem explants was greatest during dry storage of the bulbs at 17°C and 5°C, but the ability to produce shoots was lost once rapid extension growth and greening of the floral stem had commenced. Small explants, 1mm in thickness, gave rise to many adventitious buds, but larger explants (3–5mm) were optimal for maximum shoot production. There was an interaction between floral stem explant size and origin as regards shoot production. The results of a histological study of tulip shoot production in vitro are discussed. | |
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