|Authors: ||M.F. Khalilsaraie, N.T. Meti, G.S. Karibasappa|
|Keywords: ||activated charcoal, in vitro regeneration, micropropagation, multiplication |
The magnitude of demand for planting material in grape, mainly for rootstock genotypes indicates that micropropagation is inevitably necessary for their mass scale propagation, which has been a well-established methodology for in vitro regeneration of grapevine (Vitis vinifera L.). Here we present an efficient and optimized protocol for rapid in vitro regeneration of grapevine cultivar ‘Thompson Seedless’ through nodal segment culture by comparing the effect of three different cytokinins 6-benzylaminopurine (BAP), thidiazuron (TDZ) and zeatin on shoot multiplication.
Nodal segments were initiated on full, ¾ and ½ strength Murashige and Skoog’s media with or without 0.2% activated charcoal in order to study the effect of salt strength and activated charcoal on shoot multiplication.
The results indicated that BAP at all different concentrations (0.5, 1 and 2 mg/L) is responsible for shoot multiplication in grape on both ½ and ¾ strength MS medium without activated charcoal while induction of friable whitish callus and single shoot proliferation were observed in the presence of other cytokinins (zeatin and TDZ) in non-activated charcoal containing media.
In the presence of activated charcoal single shoot elongation and rooting from primary cultures are observed without callus induction.
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