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ISHS Acta Horticulturae 1083: VIII International Symposium on In Vitro Culture and Horticultural Breeding

CALLUS AND PLANTLETS PRODUCED FROM IN VITRO CULTURE OF ANTHERS OF ACTINIDIA CHINENSIS

Author:   J.-H. Wu
Keywords:   breeding, dihaploid, genetics, genomics, haploid, kiwifruit, pollen
DOI:   10.17660/ActaHortic.2015.1083.12
Abstract:
Homozygous genotypes are valuable for genomic and genetic studies in higher plants, but obtaining homozygous perennial plants using conventional breeding technologies is challenging, with the long times and large field areas required for several back-crossed generations. It is especially difficult with Actinidia because of dioecy, long generation times, high heterozygosity and variation in ploidy from diploid to octoploid. In vitro androgenesis through anther or microspore culture offers a route to develop the haploid and dihaploid plants often used in modern crop breeding, genetics and genomic studies. I report in vitro culture of anthers of six genotypes of diploid Actinidia chinensis resulting in production of callus and regeneration of plantlets. Preliminary results showed that genotype, plant growth regulators, the stage of microspore development and pre-treatments (cold, heat shock) all affected callus formation. Confirmation of their callus origin, homozygosity and determination of the ploidy levels of plantlets are underway.

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