|Authors: ||I.A. Astarini, V. Claudia, N.K.A.P. Adi, S.K. Sudirga, N.P.A. Astiti|
|Keywords: ||embryo rescue, Knudson C, W3 media, organic media, coconut husk|
Black orchid is an epiphytic orchid found mainly in Borneo Island, Indonesia.
This orchid currently is facing a large conservation problem and threatened to extinction due to human exploitation.
It is therefore an urgent requirement to develop efficient propagation technique to conserve this species.
This research aims to determine growth response and the ability of black orchid seeds to grow in different medium and to establish the best media for acclimatization.
The experiment was divided into 2 stages, seed growth in vitro, and acclimatization.
Three different media were employed during seed growth in vitro, i.e.
Western 3 media (W3), Knudson C media (KdC) and organic media (O). Each treatment consists of eight replicates.
Acclimatization stage used 4 types of media; moss, fern stem, charcoal and combination of charcoal and coconut husk.
Each consists of 8 replicates.
Results shows that seeds started to grow at 3 weeks after sowing in W3 media, and 5 weeks after sowing in other medium.
At 12 WAP, seed growth reached phase 5 (third leaf formed) on W3 medium, whilst on KdC and organic media, reached phase 4 (second leaf formed embryo). Within 3 months, the highest shoot produced was on W3 media (581 shoots), whilst on KdC and organic media produced 255 and 191 shoots respectively.
Almost all plantlets (98%) survived during acclimatization period.
Plantlets grow best on moss, compared to other media.
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