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ISHS Acta Horticulturae 1039: II International Symposium on Plant Cryopreservation

PLANT SHOOT TIP RESPONSE TO TREATMENT WITH PLANT VITRIFICATION SOLUTION # 2

Authors:   G.M. Volk, A.D. Henk, R.M. Bonnart, A. Shepherd, B.L. Gross
Keywords:   cryopreservation, gene expression, liquid nitrogen
Abstract:
Plant cryopreservation has become an effective method to conserve desirable genotypes of vegetatively propagated plant species. In many cases, excised shoot tips are treated with cryoprotectant solutions to prevent the formation of lethal ice during the freezing process. Plant vitrification solution 2 (PVS2) is a highly effective cryoprotectant solution that desiccates, permeates, and changes the freezing properties within shoot tips. We summarize a series of experiments that have been performed to better understand the mechanism by which PVS2 interacts with plant cells. Our program has measured toxicity, calorimetric, structural, and genetic responses to PVS2 and LN treatments within the shoot tips of model systems. Despite the toxicity of PVS2, it remains a highly effective vitrification solution for plant shoot tips systems.
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