|Authors: ||R. Giwanon, P. Limsiriwong, S. Nakakaew, S. Rungsri, A. Taraphan , T. Srisom |
|Keywords: ||methicillin-resistant, antibacterial activity, ethanolic extracts, diffusion method|
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infection.
Nowadays, the use of medicinal plants to treat MRSA infection has been receptive.
The aim of this study was primarily to investigate the antibacterial activity of 6 ethanolic extracts (Morus alba L., Sapindus rarak DC., Aegle marmelos (L.) Correa ex Roxb., Albizia myriophylla Benth., Blumea balsamifera (L.) DC. and Glycyrrhiza glabra L.) against 2 strains of methicillin-sensitive Staphylococcus aureus (MSSA) and 3 strains of MRSA, which were relevant to skin infection by using disc diffusion method (16 mg/disc). Among tested extracts, F extract possessed significantly the strongest antibacterial effect against both the MSSA and MRSA strains.
The range of 2 strains MSSA inhibition zones and 3 strains MRSA inhibition zones of the extract were 11.53-13.79 and 13.21-13.79 mm, respectively.
The efficacy of E and D extracts demonstrated moderate antibacterial activities against the 5 tested bacterial strains.
Weak antibacterial actions against the 5 pathogens were found in 2 ethanolic extracts, B and C extracts.
Thus, the F extract was selected to compare its potency with 2 antibiotic drugs, vancomycin (30 µg/disc) and oxacillin (1 µg/disc), against the 2 MSSA and the 3 MRSA using the same method.
Its activity against 5 bacterial strains was as effective as vancomycin.
The minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of the extract were 0.5 and 1 mg ml-1, respectively.
These findings indicate the possibility to use the F extract for the topical treatment of skin disorders relevant to MSSA and MRSA.
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