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ISHS Acta Horticulturae 1023: International Symposium on Medicinal and Aromatic Plants

ENHANCEMENT OF TNF-α INDUCED ANTIPROLIFERATION IN HUMAN CERVICAL CANCER CELLS BY EXTRACTS FROM THAI MEDICINAL PLANTS

Authors:   C. Managit, P. Sithisarn, S. Jarikasem, H. Sakurai , I. Saiki
Keywords:   Amomum biflorum, apoptosis, HeLa cells, cell viability, WST-1 cell proliferating assay
DOI:   10.17660/ActaHortic.2014.1023.28
Abstract:
Human cervical cancer is known to be a dreadful disease with high resistance to chemotherapeutic drugs in clinical use. Natural products are valuable sources for development of anticancer agents. Many plant-derived anticancer compounds were reported to modulate signal transduction pathway associated with cell proliferation and apoptosis. One well-known pathway is via the enhancement of TNF-α, a cytokine that can induce apoptotic cell death and inhibit tumorigenesis. The purpose of this study is to evaluate TNF-α antiproliferative synergistic activity of Thai medicinal plant extracts. The antiproliferative effects were determined in human cervical cancer (HeLa) cells using WST-1 cell proliferating assay. Among twenty six different tested extracts at the concentration of 30 g ml-1, 95% ethanolic extract from stems of Derris scandens, aqueous extract from leaves of Azadirachta indica, aqueous extract from leaves of A. indica var. siamensis and 95% ethanolic extract from roots of Amomum biflorum significantly decreased viability of HeLa cells treated with TNF-α (20 ng/ml). However, only 95% ethanolic extract from roots of A. biflorum combined with TNF-α significantly decreased viability of HeLa cells to 70.962.25% cell viability, when compared to cell viability of HeLa cells treated with only plant extract (93.8410.73%). Data suggested that this plant extract enhanced TNF-α antiproliferative activity in HeLa cells and it has potential for further development as chemotherapeutic agent for anticancer therapy via the enhancement of TNF-α induced apoptosis of cancer cells.

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