|Authors: || Jie Li , Yike Gao |
|Keywords: ||aneuploid, interspecific hybridization, style length, tetraploid, triploid |
More than half of Lilium in the world originate from China.
Wild Lilium of China have characteristics of strong adaptive, wide distribution and wide variations.
In order to introduce cold tolerance and disease resistance from wild species of North China (Lilium lancifolium, Lilium pumlium, Lilium concolor var. buschianum and L. davidii var. unicolor) into the Asiatic lily, conventional crossing method was used.
Several techniques, the cut-style method and KCl treatment, were developed to overcome pre-fertilization barriers.
There was a big difference in reciprocal cross. Lilium lancifolium, whose spore mother cell produce aneuploid, was not suited for being male parent and was used as female parent.
The key to success of crossing is the match between style length and pollen tube length.
When Lilium concolor var. buschianum was used as male parent, conventional stigma pollination method could not get fruits at all.
However, cutting style method was higher by 80.45 and 8.35% comparing with contrast trial which can improve fruit-bearing rate and embryo rate of seeds remarkably respectively.
Triploid and tetraploid are considered to be important materials in breeding.
The number of chromosome of hybrid of Lilium lancifolium (3n=3x=36) and ‘Loreto’ (2n=4x=48) change from 36 to 47, which is proved to be aneuploid, and Lilium lancifolium and ‘Cheops’ (2n=4x=48) change from 36 to 44. The newly developed aneuploid genotypes would greatly increase the opportunities of genetic variation, and could broke new ground on further breeding.
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